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TechnologyBioinformatics 
Cloning
EXPRESSION
Purification
Crystallisation
Structure
Expression trials are attempted first in small-scale (1ml) cultures, with different cell lines (BL21/C43), culture temperatures, induction method (IPTG v autoinduction), and medias (TB, GS9). Small scale cultures are then lysed using “bug buster”, and the presence of soluble protein is checked by a Ni/His-tag purification by the Qiagen robot, followed by a SDS gel. 
Successful expresion trials are then scaled-up to 1 litre flasks. |
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